Preparation Differential Culture Medium for Cryptococcus Neoformans from Aqueous Extract of Leaves And Flowers Of Chrysanthemum Cinerariaefolium

Neeran Obied Jasim

A new medium was prepared to isolate and diagnose the yeast Cryptococcus neoformans from flower and leaves aqueous extract of chrysanthemum. Methods Prepared differential culture medium for C. neoformans from aqueous extract of leaves and flowers of Chrysanthemum cinerariaefolium and chemical, spectral tests of the extracts were tested, in addition of gas chromatography (GC)–mass was used to diagnose phenolic compounds in both leaves and flowers. Results Showed that the yeast was grow with typical colonies on the new medium compared with other media which using in diagnosed of this yeast such as Staib agar and Sabourauds dextrose agar and unlike the yeast Candida albicans (as a negative control), which appeared in cream to white on this medium. Furthermore, the colonies are dark brown in color on flower chrysanthemum medium and light brown color on leaves chrysanthemum medium.